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Nuclei were pelleted by centrifugation (650 g, 5 min), supernatant was discarded and the pellet was resuspended in 100 l NUN1 buffer containing 20 mM Tris-HCL (pH = 7.5), 75 mM NaCl, 0.5 mM EGTA, 50 % glycerol, 1x protease and 1x phosphatase inhibitors

28: The lancet 344 :796-798,1994 29: Nutrition reviews 54 :1-30,1996 30: clinical science 91 :575-582, 1996 31: Clinical science 91 :575-582, 1996 32: Hong SY, Gil HW, Yang JO, Lee EY, Kim HK, Kim SH, Chung YH, Hwang SK, Lee ZW (2005)

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