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During ischemia, aerobic respiration is suppressed, and hypoxia drives anaerobic respiration, reducing oxidative reactions and increasing lactate release

CFTR is thought to be involved in sperm capacitation and osmoregulation via interactions with ion and water channels

Crypts ( N = 500) per well were seeded in 50 l Matrigel (BD, 356231) on a pre-warmed 24-well plate and allowed to solidify for 10 min at 37 C, after which 500 l IntestiCult Growth Medium supplemented with 100U/ml penicillin and 100 g/ml streptomycin (Biochrome, 0257F) was added