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10.1371/journal.pone.0004535 68 MairW.PiperM

To validate the methods feasibility, we selected the marketed covalent inhibitors afatinib (for lung cancer) and ibrutinib (for lymphoma) as test compounds, assessing their binding ability to GSH using this approach

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On the other hand, the pronounced changes in -SNAP-PM binding, with no significant changes in its binding capacity to intracellular membranes or LM-LUVs, reinforce the notion that the plasma membrane, with its particular biochemical and biophysical properties, constitutes a lipid environment distinct from intracellular (light) membranes 40,41,42,43,44 , and suggest that lipid composition and/or membrane biophysics also play a role in -SNAP-membrane interactions