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The cells were cultured for another 24 h and then the media were replaced with fresh MEM with 10% CCK8 solution and incubated for an extra 2 h before determining absorbances spectrophotometrically at 450 nm

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Chronobiol Int 29(2):203210 Leung M, Tranmer J, Hung E, Korsiak J, Day AG, Aronson KJ (2016) Shift work, chronotype, and melatonin patterns among female hospital employees on day and night shifts

Failure of these systems results in the accumulation of misfolded or aggregated proteins, organelle dysfunction, cellular injury, and disease progression across a wide spectrum of conditions, including neurodegeneration, cancer, cardiovascular and metabolic diseases