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Superoxide dismutasesa review of the metalassociated mechanistic variations

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Consequently, the target ion ([M-H] - ) was predefined at m / z 574

8 units/mL PreScission protease (Cytiva, Tokyo, JP, 27084301#) in potassium-Tris buffer (50 mM TrisHCl [pH 7.5], 100 mM KCl, and 1 mM dithiothreitol) was used to remove the GST tags from the EWS or RGG3, which was then add to the glutathione agarose for 16 h at 4 C