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We monitored the nuclear and overall GSH levels during cell division for over 24 h

[DOI] [PMC free article] [PubMed] [Google Scholar] Steinberg D, Parthasarathy S, Carew TE, Khoo JC, Witztum JL

Growth rates were predicted by setting the biomass reaction in the iCHO2291 model as the objective function and applying enzymatic capacity constraint flux balance analysis (ecFBA), which, unlike conventional FBA (e.g., FBA, FVA, pFBA), integrates enzyme kinetic constraints like turnover numbers ( k cat ) alongside standard FBA constraints, enhancing the accuracy of intracellular flux distributions by accounting for enzyme efficiency and enabling exploration of metabolic resource allocation 33

Conversely, reducing TSC2, a mTORC1 inhibitor, decreased pigmentation