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Two-choice preference assays A two-choice preference assay was conducted as previously described 31,56 , with minor modifications

Use one suppository every other evening before bedtime, fox six months, or as directed by a healthcare professional

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Steps include: - Prepare coffee in plunger - Allow to cool - Set your space - Pour coffee into enema bag - Insert the hose tip - Unclamp and allow coffee to flow in - Ideally hold for 12-15 minutes - Sit on toilet to release Once finished, rinse enema bag with water, add cleaner and hang to dry