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J Biol Chem 285:1296112970

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Then, combine the cooled lye solution with the oil mixture, add any special ingredients like clay minerals or herbs, and blend the solution for about 15 minutes until it thickens

The -glutamyl moiety of the donor molecule is bound to the active site at the bottom of the pocket by an extensive network of hydrogen bonds and salt bridges formed mainly with the residues present in the small subunit (Okada et al., 2006)