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(1999), which demonstrated that CEdG can induce single-strand DNA breaks and may alter DNA topology under experimental conditions 20

Buthionine sulfoximine (BSO), 1, 3-bis (2-chloroethyl)-1-nitrosourea (BCNU), diamide, and Hoechst 33342 were purchased from Sigma (St

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However, despite all these differences there is still a biological plausibility of a link between B 12 and depression given that B 12 is a necessary co-factor for methionine synthesis which provides methionine, the precursor of S-adenosylmethionine that is then needed for the formation of important brain neurotransmitters such as dopamine and norepinephrine (Reference Bailey, Stover and McNulty18,Reference Bottiglieri and Reynolds19)