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This site-specific conjugation strategy, targeting the hinge cysteine residues, enables the generation of a highly homogeneous ADC population with a controlled DAR of 23, as characterized by hydrophobic interaction chromatography (HIC), minimizing the heterogeneity and aggregation risks common with stochastic conjugation methods [54]

Brains were dissected from embryos and stored in ice cold HBSS (Sigma Aldrich)

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Therefore, when thiamine enters the cell, it cannot be used effectively 4