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The fluorescence reading was performed on the GloMax Explorer plate reader (Promega) using excitation and emission filters of 520 nm and 580 to 640 nm (green), respectively

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After three further washes, peroxidase activity was visualized using diaminobenzidine chromogen (Dako), and slides were lightly counterstained with hematoxylin before dehydration and mounting in DePex (VWR International, Oslo, Norway)