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2.9 Cell culture and treatment Mouse colon epithelial cells (MCECs) were cultured in high-glucose Dulbeccos Modified Eagle Medium, supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (P/S), in a humidified incubator with 5% CO 2 at 37C

The complexity deepens when we consider that the majority of AD patients, especially in the elderly population, carry at least one of these vascular risk factorsapproximately 65% of individuals aged 65 and 80% of those aged 85 (Lokovic et al