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Rb1 may inhibit TGF-1/Smad and ERK signaling pathways, activate Akt pathway, restore cardiac/mitochondrial function, and improve myocardial fibrosis (Figure 4

After incubation, the blots were washed and incubated with anti-mouse HRP conjugate (Cell Signaling Technology, CST7076S, RRID: AB_10956588) diluted 1:6000 in blocking buffer