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Most of the vector module libraries, including the promoter, enhancer, intron, episomal origin of replication, expression level aspect of antigen, bacterial origin and bacterial marker, can be assayed by flow cytometry to select individual human tissue culture cells that contain the reassembled (&/or subjected to one or more directed evolution methods described herein) nucleic acid sequences that have the greatest improvement in the desired property

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This suppression of the inflammatory tumor microenvironment impedes tumor growth

Add one bead volume of Blocking/Elution Buffer, mix well by pipetting several times, and incubate at room temperature for 5-10 minutes to elute the bound biotinylated molecule from the magnetic beads