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These include enzymatic antioxidants such as superoxide dismutase (SOD), catalase, and glutathione peroxidase, which neutralize ROS, and non-enzymatic antioxidants such as vitamin C, vitamin E, and glutathione, which scavenge free radicals [15]

Lysates were boiled at 95 C for 5 min, 20 g of total protein were subjected to separation by a 10% SDS gel, transferred onto nitrocellulose membranes (Amersham Biosciences) and blocked with 5% milk powder in PBS Tween (0.1%) for 1 h

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Non-targeted metabolomics was performed using a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific, MA, USA)