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A common protocol, for example, is to begin with about 20 mg of NAD+ (corresponding to a small volume from the vial, typically around 0.1 mL depending on concentration) administered subcutaneously and gradually increase the dose over subsequent injections up to around 100 mg per injection

Annu Rev Pharmacol Toxicol 18:5779 Gomez-Sintes R, Bortolozzi A, Artigas F, Lucas JJ (2014) Reduced striatal dopamine DA D2 receptor function in dominant-negative GSK-3 transgenic mice