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Cells were cultured in 200 L Neurobrew (Milteny Biotec, Bergisch Gladbach, Germany), 100 L N2-supplement (Thermo Fisher Scientific, Waltham, MA), 2 L bFGF (PeproTech, Princeton, NJ) and 2 L PDGF-BB (R&D Systems, Minneapolis, MA) per 10 mL of DMEM/F-12 media (Thermo Fisher Scientific, Waltham, MA)

Also, one study 12 reported only mild AEs with ALC (dry mouth, n=1

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Importantly, glycine is required for the synthesis of antioxidant glutathione (GSH), which enables cancer cells to cope with oxidative stress in a hypoxic microenvironment, and provides a defense mechanism against many therapeutic agents