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In brief, 8 10 5 NHDFs were embedded into a collagen I matrix (Biochrom, Berlin, Germany) and submerged cultured in construct growth medium (CGM: DMEM/F12 + GlutaMax supplemented with 10% FCS, 1% Pen/Strep, 40 M adenin HCl monohydrate, 30 g/L amphotericin B, 0.1 nM choleratoxin, 10 g/L EGF, 3.5 mg/L hydrocortisone, 4.4 mg/L insulin, 0.5% non-essential amino acids, 4.4 mg/L transferrin, and 2 nM triiodothyronin)

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These JARID1B-positive melanoma cells gave rise to a highly proliferative progeny, and knockdown of JARID1B led to accelerated tumor growth, which was followed by exhaustion