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Ltd, Tokyo, Japan) equipped with a Peltier-controlled cell holder (model PTC-517, JASCO)

You dont always want mitochondrial biogenesis

In addition, the day before running the assay, the Seahorse XF Sensor Cartridge was hydrated with sterile water followed by the calibration step (200 L per well of Seahorse XF Calibrant Solution) the day of the experiment in a non-CO 2 37 C incubator