Use the chart above to fix the volume, then draw it from the bacteriostatic water vial
(13)Fmoc-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- (13) Preparation of Fmoc-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin: (12)Fmoc-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-1050530105010603DMF3DMF2DMFFmoc-Pro-OHDIEATBTU/HOBtHBTU/HOBtBOP/HOBt105010603DMF3DMF2Fmoc-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- In the Fmoc-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin of step (12), add decapping reagent 1050 React at for 5-30 minutes, drain with a vacuum pump, re-add capping reagent at 10-50C, react for 10-60 minutes, drain, wash with isopropanol for 3 times, DMF for 3 times, and distilled DMF for 2 times , add the mixture of Fmoc-Pro-OH, DIEA, TBTU/HOBt or HBTU/HOBt or BOP/HOBt dissolved in DMF, react at 10-50C for 10-60 minutes, dry with a vacuum pump, and wash with isopropanol for 3 times , washed 3 times with DMF, washed 2 times with DMF steamed, and dried to obtain Fmoc-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala- Gly-Leu-Val-resin
The Q227E mutant displayed substantial ADH activity compared to the Wt enzyme with a K m for arogenate of 3.3 mM, similar to that of Q227E for prephenate (2.7 mM, Figure 4C, Table 1) though still 10-fold higher than that of the Wt enzyme for prephenate (Figure 4D, Table 1) and other previously characterized TyrA a enzymes (Bonvin et al., 1 s 1 , Figure 3)
Peptides do not have a one-size-fits-all dose
