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Also the mechanism of substrate-induced D2 degradation is strongly or completely impaired by replacement of Sec by Cys or Ala, respectively (88), suggesting that modification of this Sec residue during catalysis may be an essential step in the inactivation of the enzyme

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The majority of intracellular proteins are degraded by the ubiquitinproteasome system (UPS), a multi-enzyme process that involves the covalent conjugation of ubiquitin to a substrate protein and its recognition and degradation by the core multicomponent proteolytic complex of the UPS, the proteasome

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