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However, titration with His, which forms 2: 1 complex in our experimental conditions and leads to fast demetallation of CuHSA, yielded K D equal to 34.7 4.5 fM (50 mM Hepes, 50 mM NaCl at pH 7.4)

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This is the Year Things Change The essential message is clear: while the law currently prevents injectable B12 from changing its legal status, we can and must change the standard of care