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The two cysteine residues (positions 7 and 14) form an intramolecular disulfide bond that constrains the molecule into a small loop, mirroring the corresponding region of native human growth hormone (residues 177191) with an N-terminal Tyr added for synthesis and labeling reasons

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Cell proliferation IMR90 (4 10 4 ) were plated in a 24-well plate and placed onto the stage of the Incucyte Live Content Imaging instrument

Storage Store in a cool, dry place away from direct sunlight and excessive heat