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Although ultracentrifugation can separate LDL with high resolution, this method has low throughput and may cause overlap of LDL subclasses as well as destructive alterations due to the centrifugal forces as recently summarized by Kanonidou ( Gradient gel electrophoresis GGE enables the prepared electrophoretic gel to form pore gradients from large to small, so that each component in the sample can pass through the gel with a decreasing pore size during the electrophoresis process, in order to obtain better separation ( Two-dimensional gel electrophoresis (2D-GE) is also used to separate LDL particles

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