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Skin tests, in particular, are considered the gold standard for diagnosing allergic disorders

Briefly, C18 columns were activated by washing once with 400 L of acetonitrile (Fisher Scientific) and twice with 400 L of 0.1% formic acid (Thermo Scientific) in water solution, LCMS grade (Thermo Scientific)

Cells were subsequently lysed using a suitable lysis solution including protease and phosphatase inhibitors to avert protein breakdown and maintain enzymatic activity