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Specific numbers from animal studies include: reported upregulation of growth hormone receptor expression in tendon fibroblasts, effects on VEGF-mediated tube formation in endothelial cell assays, and dose-dependent reductions in gastric ulcer area in rat models at doses ranging from 10 micrograms per kilogram to higher concentrations

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it will not be effective in cases of primary ovarian failure or primary testicular failure where the glands cannot respond

For immunostaining, sections were blocked with 5% BSA + 0.3% Triton X-100 at room temperature for 30 min, then incubated overnight at 4 C with primary antibodies (GFAP: Invitrogen 130300, USA