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After 6 and 12 months, growth rates, IGF-I, and GH-binding protein (GH-BP) levels increased similarly in all groups, indicating that GH timing does not affect treatment efficacy ( Exploring whether metabolic effect of growth hormone is equally beneficial with morning versus evening growth hormone (GH) injections in GH-deficient patients is crucial for tailoring optimal treatment plans for metabolic stability and overall health

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The enzymatic activity of subcellular fractions (total, cytosol, or cellular membranes) isolated from MCF7-GSTP1 and MCF7-vector cells was evaluated using the CDNB assay