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NOV9 Sequence Analysis SEQ ID NO: 41 13040 bp NOV9a, ACAATGATGGGGCTCTTCCCCAGAACTACAGGGGCTCTGGCCATCTTCGTGGTAGTCA CG I 10477-01 TATTGGTTCATGGAGAATTGCGAATAGAGACTAAAGGTCAATATGATGAAGAAGAGAT DNA Sequence GACTATGCAACAAGCTAAAAGAAGGCAAAAACGTGAATGGGTGAAATTTGCCAAACCC TGCAGAGAAGGAGAAGATAACTCAAAAAGAAACCCAATTGCCAAGATTACTTCAGATT ACCAAGCAACCCAGAAAATCACCTACCGAATCTCTGGAGTGGGAATCGATCAGCCGCC TTTTGGAATCTTTGTTGTTGACAAAAACACTGGAGATATTAACATAACAGCTATAGTC GACCGGGAGGAAACTCCAAGCTTCCTGATCACATGTCGGGCTCTAAATGCCCAAGGAC TAGATGTAGAGAAACCACTTATACTAACGGTTAAAATTTTGGATATTAATGATAATCC TCCAGTATTTTCACAACAAATTTTCATGGGTGAAATTGAAGAAAATAGTGCCTCAGAC TCACTGGTGATGATACTAAATGCCACAGATGCAGATGAACCAAACCACTTGAATTCTA AAATTGCCTTCAAAATTGTCTCTCAGGAACCAGCAGGCACACCCATGTTCCTCCTAAG CAGAAACACTGGGGAAGTCCGTACTTTGACCAATTCTCTTGACCGAGAGCAAGCTAGC AGCTATCGTCTGGTTGTGAGTGGTGCAGACAAAGATGGAGAAGGACTATCAACTCAAT GTGAATGTAATATTAAAGTGAAAGATGTCAACGATAACTTCCCAATGTTTAGAGACTC TCAGTATTCAGCACGTATTGAAGAAAATATTTTAAGTTCTGAATTACTTCGATTTCAA GTAACAGATTTGGATGAAGAGTACACAGATAATTGGCTTGCAGTATATTTCTTTACCT CTGGGAATGAAGGAAATTGGTTTGAAATACAAACTGATCCTAGAACTAATGAAGGCAT CCTGAAAGTGGTGAAGGCTCTAGATTATGAACAACTACAAAGCGTGAAACTTAGTATT GCTGTCAAAAACAAAGCTGAATTTCACCAATCAGTTATCTCTCGATACCGAGTTCAGT CAACCCCAGTCACAATTCAGGTAATAAATGTAAGAGAAGGAATTGCATTCCGTCCTGC TTCCAAGACATTTACTGTGCAAAAAGGCATAAGTAGCAAAAAATTGGTGGATTATATC CTGGGAACATATCAAGCCATCGATGAGGACACTAACAAAGCTGCCTCAAATGTCAAGT ATGTCATGGGACGTAACGATGGTGGATACCTAATGATTGATTCAAAAACTGCTGAAAT CAAATTTGTCAAAAATATGAACCGAGATTCTACTTTCATAGTTAACAAAACAATCACA: GCTGAGGTTCTGGCCATAGATGAATACACGGGTAAAACTTCTACAGGCACGGTATATG TTAGAGTACCCGATTTCAATGACAATTGTCCAACAGCTGTCCTCGAAAAAGATGCAGT TTGCAGTTCTTCACCTTCCGTGGTTGTCTCCGCTAGAACACTGAATAATAGATACACT GGCCCCTATACATTTGCACTGGAAGATCAACCTGTAAAGTTGCCTGCCGTATGGAGTA TCACAACCCTCAATGCTACCTCGGCCCTCCTCAGAGCCCAGGAACAGATACCTCCTGG AGTATACCACATCTCCCTGGTACTTACAGACAGTCAGAACAATCGGTGTGAGATGCCA CGCAGCTTGACACTGGAAGTCTGTCAGTGTGACAACAGGGGCATCTGTGGAACTTCTT ACCCAACCACAAGCCCTGGGACCAGGTATGGCAGGCCGCACTCAGGGAGGCTGGGGCC TGCCGCCATCGGCCTGCTGCTCCTTGGTCTCCTGCTGCTGCTGGTGGCCCCCCTTCTG CTGTTGACCTGTGACTGTGGGGCAGGTTCTACTGGGGGAGTGACAGGTGGTTTTATCC CAGTTCCTGATGGCTCAGAAGGAACAATTCATCAGTGGGGAATTGAAGGAGCCCATCC TGAAGACAAGGAAATCACAAATATTTGTGTGCCTCCTGTAACAGCCAATGGAGCCGAT TTCATGGAAAGTTCTGAAGTTTGTACAAATACGTATGCCAGAGGCACAGCGGTGGAAG GCACTTCAGGAATGGAAATGACCACTAAGCTTGGAGCAGCCACTGAATCTGGAGGTGC TGCAGGCTTTGCAACAGGGACAGTGTCAGGAGCTGCTTCAGGATTCGGAGCAGCCACT GGAGTTGGCATCTGTTCCTCAGGGCAGTCTGGAACCATGAGAACAAGGCATTCCACTG GAGGAACCAATAAGGACTACGCTGATGGGGCGATAAGCATGAATTTTCTGGACTCCTA CTTTTCTCAGAAAGCATTTGCCTGTGCGGAGGAAGACGATGGCCAGGAAGCAAATGAC TGCTTGTTGATCTATGATAATGAAGGCGCAGATGCCACTGGTTCTCCTGTGGGCTCCG TGGGTTGTTGCAGTTTTATTGCTGATGACCTGGATGACAGCTTCTTGGACTCACTTGG ACCCAAATTTAAAAAACTTGCAGAGATAAGCCTTGGTGTTGATGGTGAAGGCAAAGAA GTTCAGCCACCCTCTAAAGACAGCGGTTATGGGATTGAATCCTGTGGCCATCCCATAG AAGTCCAGCAGACAGGATTTGTTAAGTGCCAGACTTTGTCAGGAAGTCAAGGAGCTTC TGCTTTGTCCACCTCTGGGTCTGTCCAGCCAGCTGTTTCCATCCCTGACCCTCTGCAG CATGGTAACTATTTAGTAACGGAGACTTACTCGGCTTCTGGTTCCCTCGTGCAACCTT CCACTGCAGGCTTTGATCCACTTCTCACACAAAATGTGATAGTGACAGAAAGGGTGAT CTGTCCCATTTCCAGTGTTCCTGGCAACCTAGCTGGCCCAACGCAGCTACGAGGGTCA CATACTATGCTCTGTACAGAGGATCCTTGCTCCCGTCTAATATGACCAGAATGAGCTG GAATACCACACTGACCAAATCTGG ORF Start: ATG at 4 ORF Stop: TGA at 3001 SEQ ID NO: 42 999 aa MW at 107518.8 Da jNOV9a, MMGLFPRTTGA AIFVWILVHGELRIETKGQYDEEEMTMQQAKRRQKRE VKFAKPC |CG1 10477 01 REGEDNSKRNPIAKITSDYQATQKITYRISGVGIDQPPFGIFWDKNTGDINITAIVD I Protein Seq uence REETPSFLITCRALNAQGLDVEKPLILTVKILDINDNPPVFSQQIFMGEIEENSASDS LVMI NATDADEPNH NSKIAFKIVSQEPAGTPMF LSR TGEVRTLTNSLDREQASS YR WSGADKDGEGLSTQCECNIKVKDVNDNFPMFRDSQYSARIEENILSSELLRFQV TDLDEEYTDN LAVYFFTSGNEGN FEIQTDPRTNEGI KWKALDYEQLQSVKLSIA VKNKAEFHQSVISRYRVQSTPVTIQVINVREGIAFRPASKTFTVQKGISSKKLVDYIL GTYQAIDEDTNKAASNVKYVMGRNDGGYLMIDSKTAEIKFVKNMNRDSTFIVNKTITA EV AIDEYTGKTSTGTVYVRVPDFNDNCPTAVLE DAVCSSSPSVWSARTLNNRYTG jPYTFALEDQPVK PAV SITTLNATSA RAQEQIPPGVYHISLVLTDSQ NRCEMPR *SLT EVCQCDNRGICGTSYPTTSPGTRYGRPHSGRLGPAAIG LLGL L LVAPLLL iLTCDCGAGSTGGVTGGFIPVPDGSEGTIHQ GIEGAHPEDKElTNICVPPVTANGADF MESSEVCTNTYARGTAVEGTSGMEMTTKLGAATESGGAAGFATGTVSGAASGFGAATG NGICSSGQSGTMRTRHSTGGTNKDYADGAISMNFLDSYFSQKAFACAEEDDGQEANDC L YDNEGADATGSPVGSVGCCSFIADDLDDSFLDSLGPKF KI-AEIS GVDGEGKEV QPPSKDSGYGIESCGHPIEVQQTGFVKCQTLSGSQGASALSTSGSVQPAVSIPDPLQH GNYLVTETYSASGSLVQPSTAGFDPLLTQNVIVTERVICPISSVPGN AGPTQLRGSH TM CTEDPCSR I Further analysis of the NOV9a protein yielded the following properties shown in Table 9B

Potency: In animal studies, Dihexa has been shown to be millions of times more potent than BDNF (Brain-Derived Neurotrophic Factor) at stimulating synapse formation

Pan et al., 2023

As a result, controlling oxidative stress has become a key neuroprotective strategy