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The proposed reasons for this decline include clogging of the beads from brownish-black precipitates and enzyme leakage

However, simulations can be sensitive to force field settings and are difficult to replicate under real-life conditions, limiting their clinical translation potential ( in vivo and in vitro experiments are still needed (Figure 1)

The purpose of the modeling was to answer questions posed by experiments or experimentalists and to investigate mechanisms of regulation in one-carbon metabolism