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This was added to the samples and incubated for 65 C for 4 h

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4.1.2 Carrier-Mediated Transport In order to transport substances such as carbohydrates, amino acids, monosaccharides, fatty acids, hormones, calcium ions, and choline into the brain, the various transporters present on the surface of ECs undergo conformational changes when they recognize their substrates, such as carbohydrates, amino acids, monosaccharides, fatty acids, hormones, calcium ions, and choline [131]