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S5) and the purified proteins low enzymatic activity 3.1 0.2 U/mg, determined by a superoxide dismutase assay kit (Cayman Chemical, Ann Arbor, MI), indicated that this protein is demetallated (apo-SOD1 WT ) or metallated only partially

Selmi C, Cavaciocchi F, Lleo A, Cheroni C, De Francesco R, Lombardi SA, De Santis M, Meda F, Raimondo MG, Crotti C, Folci M, Zammataro L, Mayo MJ, Bach N, Shimoda S, Gordon SC, Miozzo M, Invernizzi P, Podda M, Scavelli R, Martin MR, Seldin MF, LaSalle JM, Gershwin ME Frontiers in immunology 2014 5 371 Genome-wide analysis of DNA methylation, copy number variation, and gene expression in monozygotic twins discordant for primary biliary cirrhosis

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After drying, bound SRB was solubilized in 10 mM Tris base solution (pH 10.5), and plates were shaken for 30 min at RT