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Birth Defects Original Article Series

The QUIN branch of the KYN shunt is also considered the energy metabolism one, as it leads to the formation of NAD + and branches further, upstream of the QUIN formation, into a different pathway generating acetyl-CoA (35)

For lipid peroxidation level, Cells were treated with the fluorescent dye BODIPY 581/591 C11 (D3861, Thermofisher Scientific), which inserts into lipid membranes and allows for quantitative assessment of oxidized versus non-oxidized lipids by fluorescing green or red, respectively