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The generated tree in Newick format was loaded into the evolutionary tree builder MEGA6 (Tamura et al., 2013) and the root chosen using an outgroup from the accepted species tree (Murphy and Eizirik, 2009)

Guranowski, A., Montgomery, J

This level of procedural definition is essential for meaningful comparison between studies and for isolating the biological contribution of different regenerative agents

Palmitoylation is a reversible lipid modification where palmitate is covalently attached to cysteine residues through a thioester bond, which can significantly increase the hydrophobicity of proteins, thereby affecting their affinity for membrane structures, their membrane localization, protein-protein interactions, and signal transduction of the protein [60, 61]