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Lactate providing a cellular arrest signal fits well into the concept that T cells build an extra-lymphoid site where they persist, commit to pro-inflammatory effector functions, and promote the building of self-sustained lymphoid architecture ( Immunoregulation Through the Mitochondrial Intermediate Succinate By producing NADH, the tricarboxylic acid (TCA) cycle is a central route for oxidative phosphorylation, fueling complex I of the electron transport chain and eventually ATP generation

Medicine (Baltimore) 53:307323 Reddy S, Smith DB, Rich MM, Leferovich JM, Reilly P, Davis BM, Tran K, Rayburn H, Bronson R, Cros D, et al (1996) Mice lacking the myotonic dystrophy protein kinase develop a late onset progressive myopathy

Contamination occurs when sterile technique fails during preparation