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The knob mutation (T366Y) was introduced into pFUSE-HA(PR8)-hIgG1-Fc2, while the hole mutation (Y407T) was introduced into the pFUSE-naked-hIgG1-Fc construct either alone or in combination with the REW substitutions

Sugimoto T, Watanabe K, Yoshida S, Aino M, Furiki M, Shiono M, Matoh T, Biggs AR

To overcome this drawback, the invention provides methods for identifying those vectors in a genetic vaccine population that exhibit not only the desired tissue localization and longevity of DNA integrity in vivo, but retention of maximal antigen expression (or expression of other genes such as cytokines, chemokines, cell surface accessory molecules, MHC, and the like)

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