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NET-containing guanylated peptides can be internalized by FLSs through the TLR9 pathway, elevating the inflammatory phenotype of FLSs and upregulating the expression of major histocompatibility complex class II molecules, which subsequently produce autoantibodies by presentation to Ag-specific T-cells (168)

Samples were incubated for 1 h under microaerobic conditions with shaking

The freeze-drying and spray-drying processes used to make instant coffee dont appear to significantly affect chlorogenic acid levels, but the method of preparing fresh coffee does