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Plasma total cholesterol (Thermo Fisher Scientific, Infinity Total Cholesterol Reagent, TR13421), triglycerides (total glycerol and triglycerides) (Thermo Fisher Scientific, Infinity Total Triglycerides Reagent, TT22421), non-esterified fatty acids (NEFA) (Invitrogen, non-esterified free fatty acids (NEFA/FFA) Colorimetric Assay Kit, Thermo Fisher Scientific, EEA017), AST (Thermo Fisher Scientific, EEA003) and ALT (Thermo Fisher Scientific, EEA001) were quantified using enzymatic kits according to the manufacturers protocols

Offer plenty of water throughout the day, and incorporate water-rich foods like cucumbers, citrus, and melons

The role of signaling pathways such as the PI3K/AKT/mTOR and MAPK pathways in mediating the effects of iron overload on mitochondrial function has also been explored

For those already pregnant or those newly pregnant we recommend consulting with your doctor