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A very high prevalence of MASLD has been observed among obese individuals, which further strengthens the pathophysiological link between obesity and MASLD [37]

The resulting metabolic disturbances trigger the production of adipocin, especially TNF-, IL-6, and Monocyte Chemoattractant Protein-1 (MCP-1, also known as CCL2), to induce inflammatory mediators

Then, the medium was replaced with tracing medium (same as unlabeled DMEM medium, except that unlabeled glucose was replaced with stable isotope-labeled U- 13 C 6 -glucose (5 mM