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10.1155/2021/9278441 101 ShiZ.YaoF.ChenQ.ChenY.ZhangJ.GuoJ.et al (2024)

After 35 amplification cycles, the amplification products were divided into four aliquots for digestion with NcoI (New England Biolabs, NEB-R3193S), PstI (New England Biolabs, NEB-R3140S), both enzymes, or neither enzyme at 37 C for 30 min