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The 28-day countdown starts from the first puncture, and the vial must be discarded after that time

2 Materials and methods 2.1 Protocol and registration This systematic review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement (Supplementary Table S1

A: Before administering DOTCAL-D, ensure the solution is at room temperature, inspect for any physical changes such as precipitation, and follow strict hygiene protocols during injection to avoid contamination

Step 4: Let It Dissolve Protocols describe swirling the vial gently with a slow rotating motion rather than shaking it