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The purity and size of recombinant proteins were then analyzed by subjecting 20 l of the column eluates on a 10% Tris/Tricine SDS-PAGE following silver staining 40

L.LilleyK

Free Radical Biol medicine

Moreover, PER2 has been reported to form a trimeric complex with p53 and the oncogenic mouse double minute-2 homolog protein (MDM2), the p53s negative regulator, thereby increasing p53 stability by blocking Mdm2-dependent ubiquitination and transcription of p53 target genes 36