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Co-immunoprecipitation (Co-IP) NCM460 cells pretreated as indicated were collected and placed into 1.5 ml tubes (NEST Biotechnology, Wuxi, China) with 500 l Cell Lysis Buffer (Thermo Fisher Scientific) and 10 g primary antibody of anti-HIF1 (ab51608, Abcam) or anti-GPX4 (MABF1969, Sigma) at 4 C overnight

Glutathione participates in the plasma transport of toxins and xenobiotics mainly via the formation of glutathione S-conjugates, then excreted in the bile

In contrast, ubiquitination covalently attaches a larger 76-amino acid ubiquitin molecule to histone