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The animals were washed thrice with M9 buffer, and the GSH and GSSG profiles of the animals were determined using a COPAS Biosort flow cytometer (Union Biometrica Inc.)

METHYLATION PHYSIOLOGY The primary goal of methylation cycle is to continually provide the universal methyl donor, S-adenosylmethionine (for all cellular methyltransferase reactions) for the balanced functioning of methionine cycle