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Pires et al., 2022)

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Unlike replacement of solutions by microperfusion (from 1 mM to 100 mM glutamate), EPSCs recovered with much faster kinetics upon glutamate uncaging with a time constant of ~15 s, which is 10100 times faster than those estimated in isolated vesicles (Naito and Ueda, Figure 3 Despite this first success toward estimating the rate of glutamate transport in living synapses, there remain several critical concerns regarding the protocol

Lower levels of GSH can occur because of nonavailability of LC from food consumption, increased ROS production and oxidative stress from energy-rich diet consumption, and/or increased utilization of GSH relative to its biosynthesis