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After the sections were deparaffinized and hydrated, they were immersed in 0.01 mol/L sodium citrate buffer (pH = 6) for autoclaved antigen retrieval

Folic acid is an ingredient that participates in the process of cell division, while vitamin B6 helps regulate hormonal activity

The corrected analyte intensity was converted to concentration measurements using the linear equation generated by the calibration curve of the standards

Colonization studies demonstrate that specific commensals, such as Bacteroides thetaiotaomicron , are required for normal enteric neural development and neurotransmitter biosynthetic capacity 70 , indicating that microbial signals shape ENS architecture and baseline excitability