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Methylphenidate and venlafaxine attenuate locomotion in spontaneously hypertensive rats, an animal model of attention-deficit/hyperactivity disorder, through alpha2-adrenoceptor activation

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Sikiric P, Seiwerth S, Rucman R, Kolenc D, Vuletic LB, Drmic D, Grgic T, Strbe S, Zukanovic G, Crvenkovic D, Madzarac G, Rukavina I, Sucic M, Baric M, Starcevic N, Krstonijevic Z, Bencic ML, Filipcic I, Rokotov DS, Vlainic J (2016) Brain-gut axis and pentadecapeptide BPC 157: theoretical and practical implications

Following antigen retrieval in citrate buffer (pH 6.0), the tissue sections were incubated with protein-blocking agent (Immunotech, Marseille, France) at room temperature for 10 min to block nonspecific antibody binding