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Promega Corporation, Madison, WI, USA) digestion according to the manufacturers instructions for phosphorylation modification analysis, which using a Titansphere Phos-TiO kit (GL Sciences, Tokyo, Japan), and desalted using ZipTip C18 pipette tips (Millipore, Temecula, CA, USA)

The published thresholds for ELF (9.8 and 11.3) and FIB4 (1.3 and 3.25) for advanced fibrosis were validated for their ability to predict both all LRE and LRE related to portal hypertension (PHTN-LRE)