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The reactions were terminated, and the enzyme kinetics of the conjugation of 1-chloro-2,4-dinitrobenzene to GSH was determined as previously described ( Histological Examination Lung tissues were collected and fixed in 4% paraformaldehyde, then embedded in paraffin using standard procedures, following by serially sectioned as previously described ( In Vitro Pull-Down Binding Assays To determine whether GSTP binds directly to HMGB1, recombinant polyhistidine-tagged GSTP (WT), GSTP (S184A), GSTP (S184D), and HMGB1 proteins were expressed in Escherichia coli BL21 (DE3) and purified through Ni-NTA spin column (Qiagen)

Some of these metabolites, such as QUE quinone methide, have the potential to cause cellular or tissue damage due to their pro-oxidant properties