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Rats were fed purified diets containing per kg: 200 g acid casein, 326 g cornstarch, 174 g glucose, 160 g palm oil, 40 g corn oil, 50 g cellulose and vitamin and mineral mix (without calcium) according to AIN-93[41]

Indeed, SH-SY5Y cells showed high GSH levels but exhibited very low GPx activity, whereas AGS cells had low GSH content but several isoforms of the GPx enzyme

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After the cells adhered to the wall, the medium was changed to serum-free medium (LONZA, Switzerland) containing 20 ng/ml macrophage colony-stimulating factor (M-CSF) (PeproTech, USA)