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Step Three: Create a Single National Weighted Average Standardized MAO Payer-Specific Negotiated Charge Across all MS-DRGs: We would create a single national weighted average across MS-DRGs of the results of Step Two, where the weights are the national Medicare transfer adjusted case counts by MS-DRG

Real-time PCR was subsequently performed by QuantiNova TM SYBR Green PCR Kit (Qiagen) in the ABI 7900 Fast Real-Time PCR System

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